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Low-Cost Methods for Molecular Characterization of Mutant Plants: Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols
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Open Access provided with a grant from the International Atomic Energy Agency.This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits.
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Keywords
- biotechnology
- Biochemical engineering
- Biological Techniques
- Crop Improvement
- DNA extraction
- Economics, finance, business & management
- Induced mutations
- Industry & industrial studies
- Low-cost assays
- Manufacturing industries
- Mutant germplasm characterization
- Nucleic Acid Chemistry
- Nucleotide variation in plant genomes
- PCR amplification
- Pharmaceutical industries
- Plant Breeding/Biotechnology
- Plants
- Technology, engineering, agriculture
Links
DOI: 10.1007/978-3-319-16259-1web: https://link.springer.com/book/10.1007/978-3-319-16259-1
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