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Protein Engineering: Methods and Protocols
This volume details basic and advanced protocols for both stages of protein engineering: the library design phase and the identification of improved variants by screening and selection. Chapters focus on enzyme engineering using rational and semi-rational approaches. Written in the highly successful
Methods in Molecular Biology
series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Protein Engineering: Methods and Protocols aims to aid scientists in the planning and performance of their experiments.
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Keywords
- CAVER
- FRESCO algorithm
- insect cell-free expression systems
- photometric screening
- Protein tunnel inspection
- solid phase agar
- split-GFP
Links
DOI: 10.1007/978-1-4939-7366-8web: https://link.springer.com/book/10.1007/978-1-4939-7366-8
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