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Advances in Single Molecule, Real-Time (SMRT) Sequencing
Adam Ameur and Matthew S. Hestand
2019
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PacBio’s single-molecule real-time (SMRT) sequencing technology offers important advantages over the short-read DNA sequencing technologies that currently dominate the market. This includes exceptionally long read lengths (20 kb or more), unparalleled consensus accuracy, and the ability to sequence native, non-amplified DNA molecules. From fungi to insects to humans, long reads are now used to create highly accurate reference genomes by de novo assembly of genomic DNA and to obtain a comprehensive view of transcriptomes through the sequencing of full-length cDNAs. Besides reducing biases, sequencing native DNA also permits the direct measurement of DNA base modifications. Therefore, SMRT sequencing has become an attractive technology in many fields, such as agriculture, basic science, and medical research. The boundaries of SMRT sequencing are continuously being pushed by developments in bioinformatics and sample preparation. This book contains a collection of articles showcasing the latest developments and the breadth of applications enabled by SMRT sequencing technology.
This book is included in DOAB.
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Keywords
- allele-specific analysis
- bone marrow cell subpopulations
- Cladobotryum protrusum
- cobweb disease
- cytochrome P450 enzyme (CYP)
- de novo assembly
- de novo genome assembly
- DNA Methylation
- full length RNAseq
- Gene expression
- Gloeostereum incarnatum
- GRCh38
- human reference genome
- human whole-genome sequencing
- insect genome
- long-read SMRT sequencing
- low-input DNA
- mosquito
- mRNA isoforms
- mycoparasite
- n/a
- next generation sequencing
- optical mapping
- PacBio
- PacBio single molecule real-time sequencing
- population sequencing
- protein isoforms
- secondary metabolite
- single molecule real-time sequencing
- SMRT sequencing
- Statistical methods
- Swedish population
- terpenoid
- Tricoplusia ni
- whole genome sequencing
Links
DOI: 10.3390/books978-3-03921-701-4Editions
