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Cryopreservation is the storage of cells, tissues and organs at ultra-low temperatures (−196 °C) and can be an important tool for long-term conservation of plant genetic resources. A wide range of cryogenic procedures have been developed for in vitro explants, dormant buds and non-orthodox seeds in many plant species; however, research that provides novelty, insight and advances for developing and improving new protocols in plant species is still needed. This book includes the development of new protocols, physical and chemical studies related to preservation in liquid nitrogen, and some reviews on several aspects of cryopreservation. This publication will be a useful document for the community of cryopreservation scientists.
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Keywords
- activated charcoal
- alginate
- apical shoot tips
- axillary shoot tips
- Conservation
- coral
- cryobanking
- cryopreservation
- cryoprotectant
- cryoprotectant distribution
- currants
- differential scanning calorimetry
- dormant buds
- droplet-vitrification
- Eastern Turkey Beard
- embryogenic
- Endangered species
- European chestnut
- ex situ conservation
- germplasm collections
- glass transition
- glassy state
- liquid nitrogen
- long-term conservation
- long-term preservation
- low-temperature resistance
- micropropagation
- n/a
- pear tree
- plant biodiversity
- plant genebanks
- plant genetic resources
- protein expression
- PVS2 vitrification
- raman microscopy
- Reference, information & interdisciplinary subjects
- Research & information: general
- safety back-ups
- shoot culture
- shoot tips
- Symbiodiniaceae
- thema EDItEUR::G Reference, Information and Interdisciplinary subjects::GP Research and information: general
- Toxicity
- two-step freezing
- ultra-low temperature
- V cryo-plate
- vitrification
- water crystallization
- Xerophyllum asphodeloides
- zygotic embryo
Links
DOI: 10.3390/books978-3-0365-2789-5Editions