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Advanced Techniques for the Analysis of Protein and RNA
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Proteins and RNAs are vital components of cellular machinery, playing diverse roles from structural functions to regulating signaling pathways and cellular processes. Their involvement is especially critical during development and in response to environmental stimuli, influencing growth, differentiation, immune responses, and other key biological activities. Enhancing the detection of proteins and RNAs, including mRNA and microRNA, is crucial for deepening our understanding of cellular dynamics and their roles in health and disease. Recent advancements in analytical techniques, such as mass spectrometry, immunoassays, and biosensors, have significantly expanded our knowledge of protein and RNA functions and interactions. These technologies are revolutionizing healthcare and environmental science by enabling precise diagnosis, monitoring, and treatment of various diseases, thereby improving global quality of life. In the Special Issue of Chemosensors titled "Advanced Techniques for the Analysis of Proteins and RNAs," nine studies showcase cutting-edge methodologies in biosensing, enzymatic assays, chromatography, and colorimetric and fluorescent assays. Highlights include the development of a HiBiT biosensor for YAP/TAZ proteins in cancer research, an impedimetric biosensor for biomarker detection, peptide-based assays for protein quantification, and chromatographic sensors for assessing food freshness. These studies collectively advance the field of protein and RNA analysis, offering valuable insights and methodologies poised to drive further research and innovation.
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Keywords
- Antibodies
- biosensing
- Biosensor
- Biosensors
- Bradford reagent
- chemoenzymatic assay
- detection of pollutants
- device functionalization
- Ecological risk assessment
- electrochemiluminescence
- electrochemistry
- electrophoresis
- express detection
- fast protein liquid chromatography
- fish freshness
- fluorescent dye
- Food additives
- graphene FET
- hepatocellular carcinoma
- HiBiT
- hydrothermal synthesis
- hypoxanthine
- impedimetric biosensor
- inosine monophosphate
- label-free detection
- lactoferrin
- LAMP
- Ligands
- LOD
- LOQ
- meat freshness
- MicroRNAs
- multi-endpoint toxicity test
- n/a
- Nanorods
- nucleotide salvage
- Optics
- paper-based diagnostics
- peptide aptamers
- point-of-care diagnostics
- Protein
- protein assay
- protein fluorescence
- ProteOrange
- QuDye
- RT-LAMP
- rural area
- thema EDItEUR::P Mathematics and Science
- thema EDItEUR::P Mathematics and Science::PS Biology, life sciences
- thema EDItEUR::P Mathematics and Science::PS Biology, life sciences::PSD Molecular biology
- toxic equivalent concentration
- triple-negative breast cancer
- trypsin immobilization
- trypsin-inhibition-based assay
- UV detection
- YAP/TAZ
- Zinc oxide
Links
DOI: 10.3390/books978-3-7258-1936-2Editions